EN
COOLGLOW Glow Discharge
Cryo-TEM Grid Hydrophilic Treatment, Plasma Cleaning of Sample Surface
1ICP-RF technology: room temperature treatment without damage
2 Good uniformity and repeatability
3 Convenient operation
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Product Introduction
With the increasing demand for imaging applications, it is more important than ever to use clean and consistently high-quality TEM grids.

Coolglow is a compact and user-friendly glow discharger that generates a vacuum pressure of <50Pa using a high-performance mechanical pump. It then ionizes the air using an RF power supply, causing a transition of the grid surface from hydrophobic to hydrophilic. This allows for more uniform dispersion of polar suspensions on the carbon film. The RF power supply is efficient, producing a plasma that is gentler (<15eV) and has good reproducibility, resulting in reliable and repeatable results
Product Specification

Technical Specifications

Parameters

Vacuum Pump

Rotary Vane Pump

Pumping Speed

≥2m3/h

Ultimate Vacuum

<10Pa

Working Pressure

10-100Pa

Pumping Down Time

<1Min

Vacuum Gauge

Pirani Gauge

Chamber

Ø150*90mm Scratch-resistant Quartz Glass

RF Power Supply

Adjustable from 10 to 50 W, 13.56 MHz

Operating Method

Touchscreen Control with the Control System Providing Interlock Protection Functions

Weight

~20kg

Dimension

400mm*310mm*368mm

Power Supply

100-220V AC, 50/60Hz grounded three-prong plug

Power Consuption

< 300 W

Warranty

One year

Application Cases
Mouse brain slices (square membrane)
Liposomes stained with uranyl acetate (conventional carbon support membrane)
Modified protein stained with phosphotungstic acid (conventional carbon support membrane)
After hydrophilization with COOLGLOW, the samples exhibit uniform and stable distribution, eliminating issues of sample aggregation with the dye and yielding improved staining results.